Creative Bioarray can help you regulate stem cell microenvironment, control stem cell differentiation and study stem cell heterogeneity through cell patterning technology. We aim to accelerate your research process in stem cell and regenerative medicine through this technology.

Why Cell Patterning for Stem Cell Research?

Stem cells have great potential to develop into many different cell types in well-organized spatial patterns. However, the regulation of stem cell differentiation is still a basic problem in developmental biology. Because the simple laboratory environment can't simulate the biological complexity of the primary developmental environment, the traditional culture system lacks the interaction of mechanical and molecular factors in vivo. Therefore, a new generation of culture system is needed to provide an environment between Petri dish and whole animal model.

Our cell patterning technology can provide strict control of environmental parameters and reconstruct the conditions of cell culture in vitro, so as to control the fate of stem cells. This technology combines micro nano preparation, electrochemistry, surface chemistry and photochemistry to shape the physicochemical parameters related to cells, which can accurately control cell adhesion, abscission, differentiation and cell-cell interaction, and provide a new tool for stem cell biology research.

Cell Patterning in Microfluidic Devices for Regulating Stem Cell Microenvironment

Stem cells have become the main cell source and seed cells in human early embryo research, stem cell therapy and tissue engineering repair because of their pluripotency and self-renewal ability. Traditional cell research methods are difficult to provide the complex multi-level microenvironment required for stem cell growth and differentiation, and the research results are far from the real situation in vivo.

Through our cell patterning technology in the microfluidic device, we can more truly simulate the cell microenvironment, load shear force and growth factor on a single stem cell in real time and controllably, track and observe the behavior of stem cells, reduce the reaction analysis time and reduce the consumption of stem cells and analytical reagents. The combination of micro pattern and microfluidic technology can simulate and accurately regulate various factors in the stem cell microenvironment (the distribution characteristics of various soluble biochemical factors, the characteristics of extracellular matrix, etc.), which is conducive to exploring the ideal conditions for regulating the growth and differentiation of stem cells.

Patterned Co-cultures for Controlling Stem Cell Differentiation

In co-culture, stem cells not only provide a source of target cells with pluripotent differentiation ability, but also can be used as auxiliary cells to promote tissue homeostasis, metabolism, growth and repair. Therefore, co culture of stem cells and terminally differentiated cells is very important to create complex tissues or organs. To use stem cells in tissue engineering, they must be expanded, differentiated and induced to synthesize extracellular matrix. Each of these steps can benefit from the patterned co culture method. For example, cell patterning technology can reconstruct the conditions of cell culture in vitro, allow cells to be in an environment similar to the natural tissue structure, and enable stem cells to maintain their differentiation potential during expansion. 

Our cell patterning technology can also strictly control the diffusion area of cells, so as to adjust the contractile force and shape of cells, coordinate other cells, help maintain stem cells or promote the differentiation of stem cells into target tissues. This technology can also accurately control the positioning of stem cells in cocultures, allow accurate manipulation of the degree of interaction between homotypic and heterotypic cells, and drive tissue formation.

Single-cell Patterning for Stem Cell Heterogeneity

Understanding the molecular control of cell fate is the core of stem cell research. Traditional research can only reflect the overall fate of cells, and often ignores the difference of expression information between individual cells. It is necessary to investigate the molecular and cellular behavior at the single-cell level to evaluate the self-renewal and differentiation potential of cells with single-cell resolution.

Our single-cell patterning technology can capture and locate single cells in space, and even form single cell arrays to promote cell analysis. Compared with the existing methods, the cell patterning method we provide can more effectively carry out high-throughput cell operation for subsequent genomics, transcriptomics, RNA sequencing and proteomic analysis. Single-cell analysis can reveal the diversity of the composition, physiological behavior and function of various stem cells. The study of the heterogeneity of these cells can reveal the disease mechanism and determine the therapeutic target, which will bring good news for the clinical treatment of stem cells in the future.

Get in Touch

Creative Bioarray provides customers with cell patterning customization and related detection services based on Cell Patterning, you can contact our employees directly to ask questions if you are interested in our services, please contact us for more details.