Based on the Cell Patterning, Creative Bioarray use cell patterning technology to provide you with a heart model that can accurately summarize the physiological status of human heart, and provide a comprehensive in vitro cardiotoxicity assay to accurately evaluate the cardiotoxicity of drugs, and provide detailed potential mechanism information to help you make decisions earlier in the process of drug development.

Why Cell Patterning for Drugs-induced Cardiotoxicity Detection?

Many drugs that pass through the entire drug development pipeline are commercialized and subsequently recalled from the market due to unforeseen toxicity. Among them, proarrhythmic cardiotoxicity is the most common cause of withdrawal of commercial drugs. For example, valdecoxib, a non-steroidal anti-inflammatory drug that may lead to stroke, myocardial infarction or death due to adverse cardiovascular reactions, was recalled in 2005.

Therefore, there is an urgent need for a high content in vitro system that can better predict drug-induced cardiotoxicity. In vitro, the cell patterning technology based on micro-processing can maintain the physiological and pathophysiological characteristics of cardiomyocytes, reproduce the electrical activities of cardiomyocytes, be sensitive to the cytotoxic effects that will damage cardiomyocytes, and faithfully predict human cardiotoxicity.

Fig.1 Cardiotoxicity leads to cardiomyopathy, tachycardia, heart failure, and LV dysfunction. (Gabani M, et al.,2021)

Advantages

The in vitro heart models established by us through cell patterning technology can overcome many shortcomings of the traditional model, such as the inability to fully summarize human physiology. These in vitro heart models can maintain the physiological characteristics of cardiomyocytes and show more physiological correlation than the traditional model. The control of cell-cell interaction enables the model to achieve higher and stable function in a few weeks and promote the maturation of cardiomyocytes to mature heart tissue. The new models can faithfully predict human cardiotoxicity. It is a powerful tool to clarify the potential mechanism of cell injury and predict the response to poisons in vivo.

What Can We Do?

Using cell patterning technology, we can establish a variety of in vitro heart models based on human pluripotent stem cells. These models allow accurate control of the cell microenvironment, controlled intercellular interactions can achieve stable functions within a few weeks, and modular design allows the use of supporting cell types without significantly changing the homotypic interactions of cardiomyocytes. Most importantly, the model can accurately summarize the physiological or pathological conditions of human heart, including 3D anisotropic tissue structure, orientation of the extracellular matrix network and vascularization, so as to better reproduce human physiology.

Based on the above models, we provide comprehensive in vitro cardiotoxicity assays, including HERG safety assay, QT interval prolongation assay, arrhythmogenic tendency screening, comprehensive in vitro proarrhythmic assay, calcium transient assay, impedance assay and cardiac marker assay. We focus on providing you with reliable cell culture models and thoughtful detection services to accurately evaluate the cardiotoxicity of drugs and provide detailed information on potential mechanisms.

Why Choose Us?

• Overcome the limitations of existing models.
• Accurately summarize the physiological status of human heart
• Compatible with high content imaging readings
• Scientific and reasonable scheme design
• High quality and fast service

Creative Bioarray provides customers with cell patterning customization and related detection services based on Cell Patterning, you can contact our employees directly to ask questions if you are interested in our services, please contact us for more details.

Reference:

1. Gabani M.; et al. Association of cardiotoxicity with doxorubicin and trastuzumab: A double-edged sword in chemotherapy. Cureus.2021, 13(9): e18194.