Based on Cell Patterning, Creative Bioarray can use cell patterning technology to help you establish functional in vitro skeletal muscle models. The development of these models provides an important opportunity to simulate muscle diseases. In addition, the new in vitro models can significantly increase your understanding of different cell types and their interactions during skeletal muscle formation, and promote the development of new therapies for pathological muscle.

Why Cell Patterning for Skeletal Muscle Models?

Skeletal muscle tissue has extraordinary self-regeneration ability in case of slight injury. However, when muscle volume is lost, regeneration is seriously hindered. Skeletal muscle tissue engineering (SMTE) aims to repair defective skeletal muscle. So far, SMTE has made a lot of progress, but it has not yet produced a fully functional skeletal muscle tissue structure in vitro, because in vitro muscles usually show a more immature phenotype, similar to denervated muscles.

In order to improve the function of engineered muscle, it is necessary to simulate the structure and microenvironment of skeletal muscle in vitro. A common feature of all traditional methods is the fabrication of anisotropic scaffolds to allow muscle cells to align and facilitate myogenesis. However, these methods have limitations in inducing accurate 3D spatial cell tissue. One of the cell patterning technologies, 3D bioprinting technology aims to overcome these limitations by providing high-precision cell and matrix deposition to quickly manufacture complex structures.

Fig.1 Traditional and 3D bioprinting methods to design skeletal muscle tissue. (Ostrovidov S, et al.,2019)

Applications of Cell Patterning for Skeletal Muscle Models

The typical 2D culture model of skeletal muscle cells can't fully summarize the tissue and function of living muscle tissue, which limits its application in physiological research. The development of functional 3D culture model provides an important opportunity to simulate living tissue and muscle diseases. The new in vitro models can significantly increase your understanding of different cell types and their interactions during skeletal muscle formation, and promote the development of new therapies for pathological muscle. In addition, in vitro 3D skeletal muscle models are also used in regenerative medicine, cell-based detection, biorobotics, biosensing, energy harvesting and drug screening.

Fig2. Applications of 3D in vitro skeletal muscle models. (Zhuang P, et al., 2020)

What Can We Do?

Our in vitro skeletal muscle models are manufactured by cell patterning technology of 3D bioprinting, which is mainly divided into three categories: extrusion, droplet and laser bioprinting. Our technology can reproduce the complexity of active muscle tissue by accurately locating a variety of cell types, bioactive factors and biomaterials to generate cell-based constructs with complex structure. Our technology allows us to capture complex intercellular and cellular ECM interactions, Therefore, our in vitro skeletal muscle models have great potential to bridge the gap between 2D monolayer culture and in vivo research.

Our 3D printing technology is a powerful tool for building bioengineering skeletal muscle constructs. Compared with traditional models, our 3D printing technology provides a higher degree of freedom for the development of engineered skeletal muscle tissues. The controlled patterning of cell, material and biomolecular factors makes the construction of complex, complex and repeatable structures, provides an important opportunity to simulate living tissue and muscle diseases, and can significantly increase your understanding of different cell types and their interactions in the formation of skeletal muscle.

Why Choose Us?

• Replicable and scalable heterogeneous tissue
• Precise deposition of matrix and cells to create complex structures
• Reproduce the complexity of active tissue
• Rich experience in cell patterning projects
• High quality and fast service

Creative Bioarray provides customers with cell patterning customization and related detection services based on Cell Patterning, you can contact our employees directly to ask questions if you are interested in our services, please contact us for more details.

References

1. Ostrovidov, S.; et al. 3D Bioprinting in Skeletal Muscle Tissue Engineering. Small.2019, 15(24): e1805530.
2. Zhuang, P.; et al. Bioprinting of 3D in vitro skeletal muscle models: a review. Materials & Design.2020, 193:108794