Based on Cell Patterning, Creative Bioarray can use cell patterning technology to reconstruct physiological in situ conditions for controlled in vitro stem cell culture, give full play to the potential of stem cells, and become a powerful tool in your regenerative medicine, experimental modeling of diseased organs and early embryo research.

Why Cell Patterning for Regulating Cell Differentiation?

Stem cells are an undifferentiated cell bank with unlimited or long-term self-renewal ability, and have the potential to produce differentiated and specialized offspring. They are key to many cellular processes, including development, tissue regeneration and aging, as well as the hope of curing many diseases and injuries. They are multifunctional and promising cell sources for tissue engineering and regenerative medicine.

The level of cell diffusion and cell contraction are physical factors that have been proved to affect the behavior and function of stem cells. However, in the process of conventional cell culture, the cell diffusion region and cell contractility are often uncontrollable. Therefore, for the study of the effect of cell spreading on cell function, there is a great need for a convenient method to strictly control the diffusion area and size of cells. The emergence of micro patterning technology solves this problem. Using this technology, we can reconstruct the conditions of cell culture in vitro, strictly control the diffusion area of cells, so as to regulate the contractility of cells, and even change the shape of cells, so as to regulate the differentiation of stem cells.

Fig.1 Effects of cell adhesion pattern on contractility and cell differentiation. (Gomez EW, et al.,2010)

Applications of Cell Patterning for Regulating Cell Differentiation

Stem cells are undifferentiated self-regenerative progenitor cells, which can develop into different specialized cell types. Recent evidences show that cell shape is an effective biophysical regulator of stem cell differentiation. Cell patterning is an effective method to control the fate of stem cells by regulating cell shape, and reveals important insights into how geometry in the microenvironment affects cell physiology.

The plasticity of stem cells indicates that there is great potential for therapeutic applications in regenerative medicine. Adult stem cells from various tissue sources can regulate cell differentiation through cell patterning technology to influence their microenvironment and develop into related or unrelated sources of important tissue and organ cells for replacement, and may be used in regenerative medicine, such as diabetes treatment. Heart disease and several neurological diseases.

What Can We Do?

Ideal characteristics, such as low immunogenicity, pluripotency and strong self-renewal ability, make adult stem cells an attractive cell source in regenerative medicine. However, their therapeutic application is severely limited by inefficient and uncontrolled differentiation and lack of understanding of microenvironment cues leading to steady-state regulation.

Our cell patterning technology can overcome the defects of traditional culture, which can change the chemical properties of cell culture matrix on the subcellular scale, so as to limit the location and shape of the basal region so that cells can adhere to it. Cell patterning technology allows us to reconstruct physiological in situ conditions for controlled in vitro stem cell culture, give full play to the potential of stem cells, and become a powerful tool in your regenerative medicine, experimental modeling of diseased organs and early embryo research.

Why Choose Us?

• Accurate simulation of cell microenvironment
• Highly controllable on cell differentiation
• Scientific and reasonable scheme design
• Rich experience in cell patterning projects
• High quality and fast service

Creative Bioarray provides customers with cell patterning customization and related detection services based on Cell Patterning, you can contact our employees directly to ask questions if you are interested in our services, please contact us for more details.

Reference:

1. Gomez EW.; et al. Tissue geometry patterns epithelial-mesenchymal transition via intercellular mechanotransduction. J. Cell. Biochem. 2010, 110(1):44-51.