Based on Cell Patterning, Creative Bioarray can help you establish in vitro kidney models closer to the in vivo microenvironment, or even kidney disease models by cell patterning technology, so as to overcome the defects of traditional culture, we can also provide you with a personalized test platform for nephrotoxicity to improve the efficiency of preclinical drug discovery.

Why Cell Patterning for In Vitro Kidney Models?

The only treatment for patients with end-stage renal disease is dialysis and organ transplantation, but organ transplantation is limited because the number of available kidneys is far from enough. Therefore, the research of renal disease and alternative therapy is very important. The kidney is composed of a functional unit called nephron. The tubular structures such as proximal tubules, glomeruli and blood vessels in nephron are separated and interact closely. This complex renal structure can be replicated by cell patterning technology. Therefore, using cell patterning technology to simulate human renal function in vitro is very important for the research of renal diseases and new therapies, drug screening and the discovery of nephrotoxicity.

Fig.1 The human body is affected by a variety of nephrotoxic substances. (Faria, J, et al.,2019)

Applications of In Vitro Kidney Models

The kidney is very sensitive to the toxic effects of compounds, and the nephrotoxicity of drug compounds is also the main cause of acute renal injury. However, the in vitro model commonly used to study nephrotoxicity does not fully summarize the biological function of the kidney. The functional kidney model established by cell patterning technology has the ability to effectively and efficiently test the nephrotoxicity of new compounds in preclinical stage. Therefore, the model can be developed for the following aspects:

• Study kidney disease
• Study renal physiology
• Nephrotoxicity test
• Drug screening
• Regenerative medicine

What Can We Do?

In the process of drug development, 2D cell culture is mainly used for nephrotoxicity screening, but they can't accurately reproduce the 3D microenvironment and function of adult kidney. Our cell patterning technology can simulate renal function in a more physiologically relevant way, which can not only predict the improvement of organogenesis and organoid maturation in new therapies, but also improve drug screening and nephrotoxicity discovery.

Our in vitro kidney models are manufactured by cell patterning technology of 3D bioprinting, which is mainly divided into three categories: extrusion, droplet and laser bioprinting. Our comprehensive bioprinting approach allows us to develop proprietary strategies based on your needs to build 3D kidney structures that combine cells, biomaterials and other biological factors. We used 3D printing technology to produce complex perfusable renal tubular structures. Simulating the physiological environment and summarizing the filtration, absorption and excretion of drugs by the kidney, the natural kidney like behavior is realized, which can't be realized in the traditional model.

The services we can provide you based on cell patterning technology are mainly divided into the following types:

• Provide in vitro kidney model (sphere/organoid, micro tissue, kidney chip)
• Nephrotoxicity screening
• Kidney disease models (chronic kidney disease, polycystic kidney disease)
• High throughput drug screening

Why Choose Us?

• The models can be quickly formed and ready for drug treatment and analysis
• The models have higher physiological correlation.
• Scientific and reasonable scheme design
• Rich experience in cell patterning projects
• High quality and fast service

Creative Bioarray provides customers with cell patterning customization and related detection services based on Cell Patterning, you can contact our employees directly to ask questions if you are interested in our services, please contact us for more details.

Reference:

1. Faria, J.; et al. Kidney-based in vitro models for drug-induced toxicity testing. Archives of toxicology.2019, 93(12):3397–3418.