Based on Cell Patterning, Creative Bioarray designs the most suitable in vitro liver model (the model can maintain liver-specific function and maintain long-term stability of hepatocyte function) for you, and provides a variety of research endpoints to comprehensively evaluate the hepatotoxicity of your drugs and provide detailed information on the potential mechanism of drug-induced liver injury.

Why Cell Patterning for Drugs-induced Hepatotoxicity Detection?

Fig.1 Drug induced liver injury.

Drug induced liver injury is the main cause of preclinical and clinical drug loss, black-box warning of listed drugs and acute liver failure. However, the in vivo animal tests required during preclinical drug development can only identify < 50% of human drug-induced liver injury, which is mainly due to the differences in species-specific drug metabolic pathways and the inability to accurately capture human genetic and disease background. The emergence of cell patterning technology can better control the cell microenvironment, increase the life span and repeatability of cell function in vitro, and promote our understanding of liver function and injury, which is of great significance for drug development and cell-based therapy.

Advantages

The in vitro liver model established by us through cell patterning technology can overcome many shortcomings of the above traditional models, such as limited viability, rapid decline of liver function, unsuitable for high-throughput screening, lack of gene expression and cellular mechanism required for toxicity test, etc. The new in vitro liver model can maintain cell viability and metabolic capacity, specific function can be maintained for several weeks, and shows sustained liver like cell function and good correlation with in vivo clearance. The model can provide more reliable data on the potential risk of drugs. It is a powerful tool to clarify the potential mechanism of cell injury and predict the response to poisons in vivo.

What Can We Do?

We have established a variety of in vitro liver models using cell patterning technology. These models allow accurate control of the cell microenvironment to maintain liver-specific function, and can maintain the long-term (more than 4 weeks) stability of hepatocyte function. Even if repeated administration, they can maintain the sensitivity of drug-induced liver injury prediction without losing specificity.

In addition to using endpoints such as ATP, albumin, urea, ALT and lactate dehydrogenase (LDH) to evaluate the level of liver injury, we can also provide additional endpoints (mitochondrial membrane potential, reactive oxygen species, phospholipid deposition and lipid accumulation) to comprehensively evaluate the hepatotoxicity of drugs and provide detailed information on the potential mechanism of drug-induced liver injury.

Workflow

Why Choose Us?

• Long term liver metabolism and toxicity study
• Maintain liver-specific function
• Compatible with high content imaging readings
• Scientific and reasonable scheme design
• High quality and fast service

Creative Bioarray provides customers with cell patterning customization and related detection services based on Cell Patterning, you can contact our employees directly to ask questions if you are interested in our services, please contact us for more details.